Product Description

                                   SPECIFICATION SHEET

Poduct Name: Monocloanl Antibody to Cyfra21-1                   Catalog #:   L1C00705

Description: Monoclonal Antibody to Cyfra21-1(CK19)

Specificity: Cytokeratins is a subunit of human glandular epithelia. It has 20 different types dividing into two subgroups: class I (acidic protein), class II (basic protein). Cytokeratin is a polymer composed of class I and class II keratin. Cell keratin 19 (CK19) is one of the lowest molecular weight of about 400 KDa. CK19 is widely distributed in normal tissues, as lamellar or squamous epithelium. In malignant epithelial cells,the degradation of the cell that makes a lot of CK19 fragment released into the blood, the soluble fragments  can be recognized by strains of monoclonal antibody ks19.1(epitope localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQLA) of the α-helical fragment) and BM19.21 ( epitope localized on aa. 346-359 (LADVRADSERQNQE) of the α-helical fragment) so called CYFRA21 – 1. CYFRA21-1 molecular weight of about 30000 Da. CYFRA21-1 content is rich in malignant lung cancer especially in lung squamous cell carcinoma.

Host Animal: Mouse

Immunogen: Cytokeratin19 of Mr 40 000; from cultured human MCF-7 cells

Format: Purified, Liquid

Purification: >90% pure (SDS-PAGE).  Protein A chromatography.

Concentration&Lot:  1-5 mg/ml                     Lot.:  Lot. specific

Buffer: 0.01M PB, 0.15M NaCl, pH 7.4

Preservative: None

Applications: Each laboratory should determine an optimum working titer for use in its particular application.

Recommended antibody pair for immunoassay:

Capture                       Detection

L1C00706                   L1C00705

Storage&Shelf life: Store at -20°C for 3 years. Avoid multiple freeze/thaw cycles.

For research use only.